The kit contains the following materials and tools: 2 vials of 13C-labeled analytes (lyophilized) and a user manual (with product details and applications for example LC-MS methods). This QC kit is designed to evaluate the efficiency of a user’smetabolomics method and LC-/GC-MS platform such that the analytical variation can be determined and corrected. Testing and validation of the kit has been performed in an array of matrices (e.g., urine, blood, and tissues), using different forms of chromatography (e.g., RP-LC and HILIC), ionization polarity (i.e., positive and negative), and modes of MS (e.g., DDA).